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Título : MicroRNA profiling of human primary macrophages exposed to dengue virus identifies miRNA-3614-5p as antiviral and regulator of ADAR1 expression
Autor : Diosa Toro, Mayra Alejandra
Echavarrıa Consuegra, Liliana
Flipse, Jacky
Fernandez, Geysson Javier
Kluiver, Joost
Van den Berg, Anke
Urcuqui Inchima, Silvio
Smit, Jolanda
metadata.dc.subject.*: Adenosine Deaminase
Dengue Virus
Gene Deletion
Gene Expression Regulation
Macrophages
metabolism
MicroRNAs
RNA-Binding Proteins
Transcriptome
Virus Replication
Fecha de publicación : 2017
Editorial : Public Library of Science
Citación : Diosa-Toro M, Echavarría-Consuegra L, Flipse J, Fernández GJ, Kluiver J, van den Berg A, Urcuqui-Inchima S, Smit JM. MicroRNA profiling of human primary macrophages exposed to dengue virus identifies miRNA-3614-5p as antiviral and regulator of ADAR1 expression. PLoS Negl Trop Dis. 2017 Oct 18;11(10):e0005981. doi: 10.1371/journal.pntd.000598
Resumen : BACKGROUND: Due to the high burden of dengue disease worldwide, a better understanding of the interactions between dengue virus (DENV) and its human host cells is of the utmost importance. Although microRNAs modulate the outcome of several viral infections, their contribution to DENV replication is poorly understood. METHODS AND PRINCIPAL FINDINGS: We investigated the microRNA expression profile of primary human macrophages challenged with DENV and deciphered the contribution of microRNAs to infection. To this end, human primary macrophages were challenged with GFP-expressing DENV and sorted to differentiate between truly infected cells (DENV-positive) and DENV-exposed but non-infected cells (DENV-negative cells). The miRNAome was determined by small RNA-Seq analysis and the effect of differentially expressed microRNAs on DENV yield was examined. Five microRNAs were differentially expressed in human macrophages challenged with DENV. Of these, miR-3614-5p was found upregulated in DENV-negative cells and its overexpression reduced DENV infectivity. The cellular targets of miR-3614-5p were identified by liquid chromatography/mass spectrometry and western blot. Adenosine deaminase acting on RNA 1 (ADAR1) was identified as one of the targets of miR-3614-5p and was shown to promote DENV infectivity at early time points post-infection. CONCLUSION/SIGNIFICANCE: Overall, miRNAs appear to play a limited role in DENV replication in primary human macrophages. The miRNAs that were found upregulated in DENV-infected cells did not control the production of infectious virus particles. On the other hand, miR-3614-5p, which was upregulated in DENV-negative macrophages, reduced DENV infectivity and regulated ADAR1 expression, a protein that facilitates viral replication.
ISSN : 19352727
19352735 E
metadata.dc.identifier.doi: 10.1371/journal.pntd.0005981
Aparece en las colecciones: Instituto de Investigaciones Médicas

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