Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10495/12978
Título : Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia
Otros títulos : Detection of submicroscopic infection with Plasmodium spp., using classical and molecular techniques in pregnant patients from Córdoba, Colombia
Autor : Blanquiceth Melendez, Yurany Patricia
Murillo Gómez, Óscar Javier
Maestre Buitrago, Amanda Elena
Corredor Rodríguez, Mauricio
metadata.dc.subject.*: Embarazo
Gota Gruesa
Malaria
PCR en Tiempo Real y Cuantitativa (qRT-PCR)
Plasmodium Falciparum
Plasmodium Vivax
Fecha de publicación : 2014
Editorial : Universidad de Antioquia, Facultad de Medicina
Citación : Blanquiceth Melendez YP, Murillo Gómez OJ, Maestre Buitrago AE, Corredor Rodríguez M. Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia. Iatreia. 2014 Jul-Sep;27(3):277-289
Resumen : Introduction: Gestational malaria affects both the mother and the development of her embryo or fetus. Rapid diagnosis and timely and effective treatment are required to prevent complications and deaths. Objective: To compare thick blood smear with nested PCR and real-time PCR (qRT-PCR) for the diagnosis of submicroscopic infections with Plasmodium falciparum and P. vivax. Methodology: 21 women with clinical manifestations of malaria, including both pregnant and non-pregnant, were studied in Puerto Libertador, Córdoba, Colombia. Peripheral blood specimens were obtained from all of them; umbilical cord and placenta blood specimens were taken in the pregnant ones. DNA was extracted and amplified for nested PCR or qRT-PCR. Statistical analysis was done using Graphpad PRISM and EPIDAT softwares. Results: The three techniques were satisfactory for the detection of Plasmodium falciparum and P. vivax in peripheral blood and in the umbilical cord and placenta specimens. Molecular tests were 100% sensitive and specific. Two submicroscopic cases of P. falciparum infection were detected with the two PCR techniques. Conclusion: qRT-PCR is advantageous over nested PCR because its standardization is shorter, it requires lesser infrastructure and it allows the quantification of DNA.
metadata.dc.identifier.eissn: 2011-7965
ISSN : 0121-0793
Aparece en las colecciones: Instituto de Investigaciones Médicas

Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
BlanquicethYurany_2014_CasosSubmicroscopicosPlasmodiumspp.pdf996,58 kBAdobe PDFVisualizar/Abrir


Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons Creative Commons

 


Gestión de T.I. /Sistema de Bibliotecas / Universidad de Antioquia / Cl. 67 Nº 53 - 108 - Bloque 8 Conmutador: 219 51 51- 219 51 40 bibliotecadigital@udea.edu.co Medellín - Colombia