Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10495/14689
Título : Vitamin Effect on the HIV-1 Transfer from Dendritic Cells to CD4+ T Cells
Autor : Álvarez Mesa, Natalia
metadata.dc.contributor.advisor: Rugeles López, María Teresa
Aguilar Jiménez, Wbeimar
metadata.dc.subject.*: Vitamina D
Vitamin D
HIV
VIH
Células Dendríticas
Dendritic Cells
Antígenos CD4
CD4 Antigens
Fecha de publicación : 2019
Resumen : ABSTRACT: Dendritic cells (DCs) promote HIV-1 transmission by acting as Trojan horses, capturing and transporting infectious viral particles, facilitating the infection of CD4+T cells. Vitamin D (VitD) decreases HIV-1 infection of CD4+ T cells in vitro, most likely by decreasing activation and thus the susceptibility to infection; however, it is unknown if VitD could also decrease viral transfer from DCs to CD4+ T cells. In this study, we co-cultured HIV-1-pulsed immature (iDCs) and mature (mDCs) monocytes-derived dendritic cells, differentiated or not in the presence of calcitriol (VitD active form), with VitD-untreated PHA-activated autologous CD4+ T cells from 16 healthy donors. We observed that co-cultures performed with VitD-treated iDCs and mDCs significantly decreased the frequency of infected CD4+ T cells, evaluated by flow cytometry. However, p24 levels, evaluated by ELISA, were not significantly reduced in culture supernatants. Regarding genes involved in HIV-1 transfer, in VitD-treated iDCs we observed a decreased expression of these genes compared to the control; moreover, VitD-treated iDCs and mDCs exhibit a similar gene expression profile, maybe related to a transcriptional balance achieved after a long treatment with VitD. Finally, we found that DCs differentiated with VitD reduced the surface expression of DC-SIGN and SIGLEC-1 receptors, widely associated with HIV-1 transfer, thus, it could be the most most likely mechanism by which VitD affects viral transfer.
Aparece en las colecciones: Maestrías en Ciencias Básicas Biomédicas

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