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dc.contributor.authorCamargo Guerrero, Mauricio-
dc.contributor.authorDuque Correa, María Adelaida-
dc.contributor.authorBerrío, Alejandro-
dc.date.accessioned2021-10-30T00:07:05Z-
dc.date.available2021-10-30T00:07:05Z-
dc.date.issued2006-
dc.identifier.issn0074-0276-
dc.identifier.urihttp://hdl.handle.net/10495/23665-
dc.description.abstractABSTRACT: Cytogenetics of triatomines have been a valuable biological tool for the study of evolution, taxonomy, and epidemiology of these vectors of Trypanosoma cruzi. Here we present a single microtube protocol that combines micro-centrifugation and micro-spreading, allowing high quality cytogenetic preparations from male gonadal material of Rhodnius prolixus and Triatoma lecticularia. The amount of cellular scattering can be modulated, which can be useful if small aggregates of synchronous cells are desired. Moreover, a higher number of slides per gonad can be obtained with fully flattened clean chromosomal spreads with minimum overlaps, optimal for classical and modern molecular cytogenetic analyses.spa
dc.format.extent2spa
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherInstituto Oswaldo Cruzspa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersionspa
dc.rightsinfo:eu-repo/semantics/openAccessspa
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/co/*
dc.titleA micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae)spa
dc.typeinfo:eu-repo/semantics/articlespa
dc.publisher.groupBiología y Control de Enfermedades Infecciosasspa
dc.publisher.groupGenética Regeneración y Cáncerspa
dc.identifier.doi10.1590/S0074-02762006000300021-
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2spa
dc.identifier.eissn1678-8060-
oaire.citationtitleMemórias do Instituto Oswaldo Cruzspa
oaire.citationstartpage339spa
oaire.citationendpage340spa
oaire.citationvolume101spa
oaire.citationissue3spa
dc.rights.creativecommonshttps://creativecommons.org/licenses/by/4.0/spa
dc.publisher.placeRío de Janeiro, Brasilspa
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1spa
dc.type.redcolhttps://purl.org/redcol/resource_type/ARTCORTspa
dc.type.localArtículo de investigaciónspa
dc.subject.decsTriatominae-
dc.subject.agrovocCromosoma-
dc.subject.agrovocChromosomes-
dc.subject.proposalMicrodifusiónspa
dc.subject.agrovocurihttp://aims.fao.org/aos/agrovoc/c_1598-
dc.description.researchgroupidCOL0007865spa
dc.description.researchgroupidCOL0006769spa
dc.subject.meshurihttps://id.nlm.nih.gov/mesh/D014225-
dc.relation.ispartofjournalabbrevMem. Inst. Oswaldo Cruz.spa
Aparece en las colecciones: Artículos de Revista en Ciencias Exactas y Naturales

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