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dc.contributor.authorPathmanathan, Siva Gowri-
dc.contributor.authorCardona Castro, Nora María-
dc.contributor.authorSánchez Jiménez, Miryan Margot-
dc.contributor.authorCorrea Ochoa, Margarita María-
dc.date.accessioned2022-03-12T18:10:56Z-
dc.date.available2022-03-12T18:10:56Z-
dc.date.issued2003-
dc.identifier.citationPathmanathan SG, Cardona-Castro N, Sánchez-Jiménez MM, Correa-Ochoa MM, Puthucheary SD, Thong KL. Simple and rapid detection of Salmonella strains by direct PCR amplification of the hilA gene. J Med Microbiol. 2003 Sep;52(Pt 9):773-776. doi: 10.1099/jmm.0.05188-0.spa
dc.identifier.issn0022-2615-
dc.identifier.urihttp://hdl.handle.net/10495/26543-
dc.description.abstractABSTRACT : The suitability of a PCR procedure using a pair of primers targeting the hilA gene was evaluated as a means of detecting Salmonella species. A total of 33 Salmonella strains from 27 serovars and 15 non-Salmonella strains from eight different genera were included. PCR with all the Salmonella strains produced a 784 bp DNA fragment that was absent from all the non-Salmonella strains tested. The detection limit of the PCR was 100 pg with genomic DNA and 3 3 104 c.f.u. ml 1 with serial dilutions of bacterial culture. An enrichment-PCR method was further developed to test the sensitivity of the hilA primers for the detection of Salmonella in faecal samples spiked with different concentrations of Salmonella choleraesuis subsp. choleraesuis serovar Typhimurium. The method described allowed the detection of Salmonella Typhimurium in faecal samples at a concentration of 3 3 102 c.f.u. ml 1. In conclusion, the hilA primers are specific for Salmonella species and the PCR method presented may be suitable for the detection of Salmonella in faeces.spa
dc.format.extent4spa
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherMicrobiology Societyspa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersionspa
dc.rightsinfo:eu-repo/semantics/openAccessspa
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/co/*
dc.titleSimple and rapid detection of Salmonella strains by direct PCR amplification of the hilA genespa
dc.typeinfo:eu-repo/semantics/articlespa
dc.publisher.groupMicrobiología Molecularspa
dc.identifier.doi10.1099/jmm.0.05188-0-
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2spa
dc.identifier.eissn1473-5644-
oaire.citationtitleJournal of Medical Microbiologyspa
oaire.citationstartpage773spa
oaire.citationendpage776spa
oaire.citationvolume52spa
oaire.citationissuePt 9spa
dc.rights.creativecommonshttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.publisher.placeLondres, Inglaterraspa
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1spa
dc.type.redcolhttps://purl.org/redcol/resource_type/ARTspa
dc.type.localArtículo de investigaciónspa
dc.subject.decsProteínas Bacterianas-
dc.subject.decsBacterial Proteins-
dc.subject.decsSalmonella-
dc.subject.decsSalmonella typhimurium-
dc.subject.decsSensibilidad y Especificidad-
dc.subject.decsSensitivity and Specificity-
dc.subject.decsReacción en Cadena de la Polimerasa-
dc.subject.decsPolymerase Chain Reaction-
dc.description.researchgroupidCOL0013746spa
dc.relation.ispartofjournalabbrevJ. Med. Microbiol.spa
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