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Título : Impact of Resistance to Fluconazole on Virulence and Morphological Aspects of Cryptococcus Neoformans and Cryptococcus Gattii Isolates
Autor : Rossi, Suélen Andreia
Trevijano Contador, Nuria
Scorzoni, Liliana
Mesa Arango, Ana Cecilia
de Oliveira, Haroldo Cesar
Werther, Karin
de Freitas Raso, Tânia
Mendes Giannini, Maria José Soares
Zaragoza Hernández, Oscar
Fusco Almeida, Ana Marisa
metadata.dc.subject.*: Fluconazole
Fluconazol
Drug Resistance
Resistencia a Medicamentos
Virulence
Virulencia
Cryptococcus gattii
Cryptococcus neoformans
Fecha de publicación : 2016
Editorial : Frontiers Research Foundation
Resumen : ABSTRACT: Cryptococcus sp. are responsible for around 1 million cases of meningitis every year. Fluconazole (FLU) is commonly used in the treatment of cryptococcosis, mainly in immunocompromised patients and the resistance is usually reported after long periods of treatment. In this study, the morphological characterization and virulence profile of FLU-susceptible and FLU-resistant clinical and environmental isolates of C. neoformans and C. gattii were performed both in vitro and in vivo using the Galleria mellonella model. FLU-susceptible isolates from C. neoformans were significantly more virulent than the FLU-resistant isolates. FLU-susceptible C. gattii isolates showed a different virulence profile from C. neoformans isolates where only the environmental isolate, CL, was more virulent compared with the resistant isolates. Cell morphology and capsule size were analyzed and the FLU-resistant isolates did not change significantly compared with the most sensitive isolates. Growth at 37◦C was also evaluated and in both species, the resistant isolates showed a reduced growth at this temperature, indicating that FLU resistance can affect their growth. Based on the results obtained is possible suggest that FLU resistance can influence the morphology of the isolates and consequently changed the virulence profiles. The most evident results were observed for C. neoformans showing that the adaptation of isolates to antifungal selective pressure influenced the loss of virulence.
metadata.dc.identifier.eissn: 1664-302X
metadata.dc.identifier.doi: 10.3389/fmicb.2016.00153
metadata.dc.identifier.url: https://www.frontiersin.org/articles/10.3389/fmicb.2016.00153/full
Aparece en las colecciones: Artículos de Revista en Ciencias Médicas

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