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dc.contributor.authorOsorio Durango, Edison-
dc.contributor.authorLara Guzmán, Oscar Javier-
dc.contributor.authorMuñoz Durango, Katalina-
dc.contributor.authorÁlvarez Quintero, Rafael Mariano-
dc.contributor.authorGil Izquierdo, Ángel-
dc.contributor.authorMedina, Sonia-
dc.contributor.authorZuluaga, Natalia-
dc.contributor.authorOger, Camille-
dc.contributor.authorGalano, Jean Marie-
dc.contributor.authorDurand, Thierry-
dc.date.accessioned2023-10-10T18:33:53Z-
dc.date.available2023-10-10T18:33:53Z-
dc.date.issued2018-
dc.identifier.citationLara-Guzmán, O. J., Gil-Izquierdo, Á., Medina, S., Osorio, E., Álvarez-Quintero, R., Zuluaga, N., Oger, C., Galano, J. M., Durand, T., & Muñoz-Durango, K. (2018). Oxidized LDL triggers changes in oxidative stress and inflammatory biomarkers in human macrophages. Redox biology, 15, 1–11. https://doi.org/10.1016/j.redox.2017.11.017spa
dc.identifier.urihttps://hdl.handle.net/10495/36865-
dc.description.abstractABSTRACT: Oxidized low-density lipoprotein (oxLDL) is a well-recognized proatherogenic particle that functions in atherosclerosis. In this study, we established conditions to generate human oxLDL, characterized according to the grade of lipid and protein oxidation, particle size and oxylipin content. The induction effect of the cellular proatherogenic response was assessed in foam cells by using an oxLDL-macrophage interaction model. Uptake of oxLDL, reactive oxygen species production and expression of oxLDL receptors (CD36, SR-A and LOX-1) were significantly increased in THP-1 macrophages. Analyses of 35 oxylipins revealed that isoprostanes (IsoP) and prostaglandins (PGs) derived from the oxidation of arachidonic, dihomo gamma-linolenic and eicosapentaenoic acids were strongly and significantly induced in macrophages stimulated with oxLDL. Importantly, the main metabolites responsible for the THP1-macrophage response to oxLDL exposure were the oxidative stress markers 5-epi-5-F2t-IsoP, 15-E1t-IsoP, 8-F3t-IsoP and 15-keto-15-F2t-IsoP as well as inflammatory markers PGDM, 17-trans-PGF3α, and 11β-PGF2α, all of which are reported here, for the first time, to function in the interaction of oxLDL with THP-1 macrophages. By contrast, a salvage pathway mediated by anti-inflammatory PGs (PGE1 and 17-trans-PGF3α) was also identified, suggesting a response to oxLDL-induced injury. In conclusion, when THP-1 macrophages were treated with oxLDL, a specific induction of biomarkers related to oxidative stress and inflammation was triggered. This work contributes to our understanding of initial atherogenic events mediated by oxLDL-macrophage interactions and helps to generate new approaches for their modulation.spa
dc.format.extent11spa
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherElsevierspa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersionspa
dc.rightsinfo:eu-repo/semantics/openAccessspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/co/*
dc.titleOxidized LDL triggers changes in oxidative stress and inflammatory biomarkers in human macrophagesspa
dc.typeinfo:eu-repo/semantics/articlespa
dc.publisher.groupGrupo de Investigación en Sustancias Bioactivas (GISB)spa
dc.identifier.doi10.1016/j.redox.2017.11.017-
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2spa
dc.identifier.eissn2213-2317-
oaire.citationtitleRedox Biologyspa
oaire.citationstartpage1spa
oaire.citationendpage11spa
oaire.citationvolume15spa
dc.rights.creativecommonshttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.publisher.placeÁmsterdam, Paises Bajosspa
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1spa
dc.type.redcolhttps://purl.org/redcol/resource_type/ARTspa
dc.type.localArtículo de investigaciónspa
dc.subject.decsAterosclerosis-
dc.subject.decsAtherosclerosis-
dc.subject.decsBiomarcadores - metabolismo-
dc.subject.decsBiomarkers - metabolism-
dc.subject.decsAntígenos CD36 - genética-
dc.subject.decsCD36 Antigens - genetics-
dc.subject.decsLínea Celular-
dc.subject.decsCell Line-
dc.subject.decsCélulas Espumosas-
dc.subject.decsFoam Cells-
dc.subject.decsInflamación-
dc.subject.decsInflammation-
dc.subject.decsLipoproteínas LDL-
dc.subject.decsLipoproteins, LDL-
dc.subject.decsMacrófagos - metabolismo-
dc.subject.decsMacrophages - metabolism-
dc.subject.decsEstrés Oxidativo - genética-
dc.subject.decsOxidative Stress - genetics-
dc.subject.decsEspecies Reactivas de Oxígeno - metabolismo-
dc.subject.decsReactive Oxygen Species - metabolism-
dc.subject.decsReceptores Depuradores de Clase E - genética-
dc.subject.decsScavenger Receptors, Class E - genetics-
dc.subject.decsFactores de Empalme Serina-Arginina - genética-
dc.subject.decsSerine-Arginine Splicing Factors - genetics-
dc.description.researchgroupidCOL0010359spa
dc.relation.ispartofjournalabbrevRedox Biol.spa
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