Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/10495/43571
Título : A strain-independent method to induce progressive and lethal pneumococcal pneumonia in neutropenic mice
Autor : Salazar Giraldo, Beatriz
Zuluaga Salazar, Andrés Felipe
Agudelo García, Olga María
Rodríguez Jaramillo, Carlos Andrés
Vesga Meneses, Omar
metadata.dc.subject.*: Farmacorresistencia Microbiana
Drug Resistance, Microbial
Pulmón
Lung
Mucinas
Mucins
Penicilinas
Penicillins
Neumonía Neumocócica
Pneumonia, Pneumococcal
Serogrupo
Serogroup
Streptococcus pneumoniae
Streptococcus pneumoniae
Modelos Animales de Enfermedad
Disease Models, Animal
https://id.nlm.nih.gov/mesh/D004352
https://id.nlm.nih.gov/mesh/D008168
https://id.nlm.nih.gov/mesh/D009077
https://id.nlm.nih.gov/mesh/D010406
https://id.nlm.nih.gov/mesh/D011018
https://id.nlm.nih.gov/mesh/D065288
https://id.nlm.nih.gov/mesh/D013296
https://id.nlm.nih.gov/mesh/D004195
Fecha de publicación : 2015
Editorial : BMC (BioMed Central)
Citación : Zuluaga AF, Salazar BE, Agudelo M, Rodriguez CA, Vesga O. A strain-independent method to induce progressive and lethal pneumococcal pneumonia in neutropenic mice. J Biomed Sci. 2015 Mar 25;22(1):24. doi: 10.1186/s12929-015-0124-4.
Resumen : ABSTRACT: Background: Experimental models of pneumonia with penicillin non-susceptible Streptococcus pneumoniae (PNSSP) are hard to reproduce because the majority of strains with clinical relevance (like serotypes 6B, 9 V and 19 F) have low murine virulence. By optimization of culture and inoculum conditions of PNSSP (using porcine mucin), our aim was to develop a suitable, reliable and reproducible pneumonia mouse model for anti-infective pharmacology research. Results: Seven PNSSP strains, including serotypes 6B, 9 V, 14 and 19 F were included. Strain INS-E611 displayed the highest murine virulence and was chosen to validate the lung model. Nose-instilled pneumococci grew between 2.1 and 2.5 log10 CFU/g of lung in 24 hours when an optimized culture of bacterial cells was used, but animals were all alive and recovered of infection after 36 h. In contrast, inoculum supplementation with mucin led to 100% mortality related to a successful lung infection confirmed by histopathology. These findings were reproduced with all seven PNSSP strains in neutropenic mice. Immunocompetent animals cleared all strains spontaneously. Conclusions: This pneumonia model produces a progressive and uniformly fatal lung infection with diverse serotypes of PNSSP independently of their intrinsic murine virulence.
metadata.dc.identifier.eissn: 1423-0127
ISSN : 1021-7770
metadata.dc.identifier.doi: 10.1186/s12929-015-0124-4
Aparece en las colecciones: Artículos de Revista en Ciencias Médicas

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