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https://hdl.handle.net/10495/43571
Título : | A strain-independent method to induce progressive and lethal pneumococcal pneumonia in neutropenic mice |
Autor : | Salazar Giraldo, Beatriz Zuluaga Salazar, Andrés Felipe Agudelo García, Olga María Rodríguez Jaramillo, Carlos Andrés Vesga Meneses, Omar |
metadata.dc.subject.*: | Farmacorresistencia Microbiana Drug Resistance, Microbial Pulmón Lung Mucinas Mucins Penicilinas Penicillins Neumonía Neumocócica Pneumonia, Pneumococcal Serogrupo Serogroup Streptococcus pneumoniae Streptococcus pneumoniae Modelos Animales de Enfermedad Disease Models, Animal https://id.nlm.nih.gov/mesh/D004352 https://id.nlm.nih.gov/mesh/D008168 https://id.nlm.nih.gov/mesh/D009077 https://id.nlm.nih.gov/mesh/D010406 https://id.nlm.nih.gov/mesh/D011018 https://id.nlm.nih.gov/mesh/D065288 https://id.nlm.nih.gov/mesh/D013296 https://id.nlm.nih.gov/mesh/D004195 |
Fecha de publicación : | 2015 |
Editorial : | BMC (BioMed Central) |
Citación : | Zuluaga AF, Salazar BE, Agudelo M, Rodriguez CA, Vesga O. A strain-independent method to induce progressive and lethal pneumococcal pneumonia in neutropenic mice. J Biomed Sci. 2015 Mar 25;22(1):24. doi: 10.1186/s12929-015-0124-4. |
Resumen : | ABSTRACT: Background: Experimental models of pneumonia with penicillin non-susceptible Streptococcus pneumoniae (PNSSP) are hard to reproduce because the majority of strains with clinical relevance (like serotypes 6B, 9 V and 19 F) have low murine virulence. By optimization of culture and inoculum conditions of PNSSP (using porcine mucin), our aim was to develop a suitable, reliable and reproducible pneumonia mouse model for anti-infective pharmacology research. Results: Seven PNSSP strains, including serotypes 6B, 9 V, 14 and 19 F were included. Strain INS-E611 displayed the highest murine virulence and was chosen to validate the lung model. Nose-instilled pneumococci grew between 2.1 and 2.5 log10 CFU/g of lung in 24 hours when an optimized culture of bacterial cells was used, but animals were all alive and recovered of infection after 36 h. In contrast, inoculum supplementation with mucin led to 100% mortality related to a successful lung infection confirmed by histopathology. These findings were reproduced with all seven PNSSP strains in neutropenic mice. Immunocompetent animals cleared all strains spontaneously. Conclusions: This pneumonia model produces a progressive and uniformly fatal lung infection with diverse serotypes of PNSSP independently of their intrinsic murine virulence. |
metadata.dc.identifier.eissn: | 1423-0127 |
ISSN : | 1021-7770 |
metadata.dc.identifier.doi: | 10.1186/s12929-015-0124-4 |
Aparece en las colecciones: | Artículos de Revista en Ciencias Médicas |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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ZuluagaAndres_2015_Strain-Independent_Method .pdf | Artículo de investigación | 2.22 MB | Adobe PDF | Visualizar/Abrir |
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