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dc.contributor.authorVásquez Palacio, Gonzalo de Jesús-
dc.contributor.authorRuiz Cortés, Zulma Tatiana-
dc.contributor.authorOlivera Ángel, Martha-
dc.contributor.authorDavid López, Diana Janneth-
dc.date.accessioned2019-07-17T16:03:19Z-
dc.date.available2019-07-17T16:03:19Z-
dc.date.issued2008-
dc.identifier.citationDavid López D, Vásquez Palacio G, Ruiz Cortés T, Olivera Angel M. Caracterización citogenética del pez neotropical Brycon henni (Pisces: Characidae). Rev Biol Trop. 2008;56(4):1619-1628spa
dc.identifier.issn0034-7744-
dc.identifier.urihttp://hdl.handle.net/10495/11450-
dc.description.abstractABSTARCT: Brycon henni, is a native fish from Western Colombia is important as food and in sport fishing, and could be cultured in waters between 18 and 28°C. A previous cytogenetic study in branchial cells indicated different chromosomal complements. Total blood samples were seeded in 4 ml Ham-F12 culture medium, supplemented with 0.5 ml Bovine Fetal Serum and 0.7 ml Phyitohemaglutinin M (Gibco®) during 96 hr at 28°C; an antimitotic factor (100μl Colcemid® 10%) was added. Cells were incubated in 10 ml KCl hypotonic solution (0.56%) for 24 min at 37°C, fixed in methanol; acetic acid (3: 1) three times, separated by centrifugations, extended drip, and the chromosomes slides were stained with 5% Giemsa (5%). The best metaphases (6 males and 8 females) were photographed. There was a diploid number of 50 chromosomes: Metacentrics (26M), Submetacentrics (16SM) and Subtelocentrics (8ST). The fundamental number is 100 arms. The evolutionary tendency is type B with no supernumerary chromosomes; a first pair of big metacentric chromosomes is characteristic for Brycon, while no chromosomic sexual heteromorfism was detected. Key words: Karyotype, chromosomes, fish, bryconids, lymphocytes.spa
dc.format.extent9spa
dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.publisherUniversidad de Costa Ricaspa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersionspa
dc.rightsAtribución 2.5*
dc.rightsinfo:eu-repo/semantics/openAccessspa
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/co/*
dc.subjectCariotipo-
dc.subjectCromosomas-
dc.subjectLinfocitos-
dc.subjectPez-
dc.subjectBryconids-
dc.subjectChromosomes-
dc.subjectFish-
dc.subjectKaryotype-
dc.subjectLymphocytes-
dc.titleCaracterización citogenética del pez neotropical Brycon henni (Pisces: Characidae)spa
dc.title.alternativeCytogenetic characterization of the neotropical fish Brycon henni (Pisces: Characidae)spa
dc.typeinfo:eu-repo/semantics/articlespa
dc.publisher.groupBiogénesisspa
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2spa
dc.identifier.eissn2215-2075-
oaire.citationtitleRevista de Biología Tropicalspa
oaire.citationstartpage1619spa
oaire.citationendpage1628spa
oaire.citationvolume56spa
oaire.citationissue4spa
dc.rights.creativecommonshttps://creativecommons.org/licenses/by/4.0/spa
dc.publisher.placeCosta Riccaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1spa
dc.type.redcolhttps://purl.org/redcol/resource_type/ARTspa
dc.type.localArtículo de investigaciónspa
dc.relation.ispartofjournalabbrevRev. Biol. Tropspa
Aparece en las colecciones: Instituto de Investigaciones Médicas

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