Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10495/12844
Título : Desarrollo de un sistema de liberación controlada de doxiciclina a partir de dos prototipos de encapsulación : secado por aspersión y liposomas
Autor : Gallego Londoño, Vanessa
metadata.dc.contributor.advisor: Londoño Londoño, Julián
Jiménez Cartagena, Claudio
Monsalve Buriticá, Santiago
Gil Garzón, Maritza Andrea
metadata.dc.subject.*: Doxiciclina
Infecciones intracelulares
Liberación in vitro
Liposomas
Macrófagos
Doxycycline
Intracellular infections
In vitro release
Liposomes
Macrophages
Fecha de publicación : 2017
Citación : Gallego V. Desarrollo de un sistema de liberación controlada de doxiciclina a partir de dos prototipos de encapsulación: secado por aspersión y liposomas [Tesis de maestría]. Medellín, Colombia: Universidad de Antioquia; 2017.
Resumen : Drug delivery systems are a promising alternative for intracellular infections treatments due to their capability to modify drug release profiles and even vectorize in a cellular level. Two drug delivery systems of doxycycline were developed in this research with microparticles and liposomes, the first one elaborated by spray drying and the second with reverse-phase evaporation vesicle. Microparticles were prepared with two different wall material mixtures: gelatin:sucrose 70:30 and gelatin:sucrose:carboxymethyl cellulose 13.64:85.86:0.50, and were evaluated in terms of moisture, water activity, flow properties, size, zeta potential, yield, efficiency and in vitro release. Two different types of liposomes were designed: neutral DMPC and anionic DMPC:DMPS 1:1, each one with 6 mg/mL of phospholipid. The DMPS addition effect on DMPC liposomes was studied by its size and surface charge, as well as the doxycycline incorporation capacity on the two systems and the liposomes internalization in DH82 cells by confocal microscopy. The microparticulate system, composed by gelatin:sucrose:carboxymethyl cellulose showed better characteristics in terms of Carr’s Index (23.33 %), Hausner Ratio (1.30), soft morphology and smooth external surface, encapsulation efficiency (57.91 %) and lower quantity of active ingredient released at acid pH. Regarding liposomes, those composed by DMPC:DMPS 1:1 showed to be mainly uptake according to the data provided by the fluorescence intensity profile. Results suggested that both formulations, microparticles and liposomes, have potential to be used in the intracellular diseases treatments in which doxycycline has therapeutical effect.
Aparece en las colecciones: Maestrías en Ciencias Farmacéuticas y Alimentarias

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