Caracterización del viroma de ARN en tejido radical de Solanum phureja mediante pirosecuenciación 454 GS-FLX

Abstract

RESUMEN: Las enfermedades virales son una de las principales limitantes para la producción de papa en la región Andina al reducir los rendimientos de los cultivos, afectar la calidad de los tubérculos y limitar su utilidad como semilla. Los programas de manejo integrado de las enfermedades virales deben estar sustentados en el conocimiento previo de las especies de virus y sus variantes presentes en los cultivares establecidos en cada región y en la disponibilidad de herramientas de diagnóstico viral específicas y altamente sensibles. En esta investigación se caracterizó el viroma de ARN asociado a tejido radicular de una planta de Solanum phureja cultivar Criolla Colombia mediante la pirosecuenciación del ADNc utilizando el sistema 454 GS-FLX. Los resultados demostraron la coinfección de tres virus de ARN en las raíces analizadas: dos variantes de Potato virus S detectadas en el 49,7 % (PVSCol) y 42,7 % (PVSA) del total de reads asociados a cápsides virales, el Potato virus V (PVV) (6,5%) y el Potato leafroll virus (PLRV) (1,1%). Adicionalmente, se identificaron al menos dos diferentes variantes genómicas para cada uno de los virus, lo que debe ser considerado para el diseño futuro de herramientas de detección molecular que apoyen los programas de certificación de tubérculo-semilla y mejoramiento genético de papa por resistencia a virus.

ABSTRACT: Viral diseases are one of the most limiting factors to the potato industry in the Andean region as they significantly reduce yields, compromise tuber quality and limit their use as seed tubers. Integrated management programs of viral diseases must be supported by a good knowledge of the virus species (and their variants) infecting each potato variety and the availability of specific and highly sensitive diagnostic tools. In this work, the root RNA-virome of a Solanum phureja cv. Criolla Colombia plant was characterized using 454 GS-FLX pyrosequencing. Co-infection with three different viruses was found, the most prevalent of which were identified as two variants of Potato virus S: PVSCol (49.7 %) and PVSA (42.7 %). The remaining viruses corresponded to Potato virus V (PVV) (6.5 %) and Potato leafroll virus (PLRV) (1.1 %). At least two genomic variants were found for each virus, an important factor that should be taken into account in the design of molecular detection tools supporting seed certification and genetic improvement programs.Viral diseases are one of the most limiting factors to the potato industry in the Andean region as they significantly reduce yields, compromise tuber quality and limit their use as seed tubers. Integrated management programs of viral diseases must be supported by a good knowledge of the virus species (and their variants) infecting each potato variety and the availability of specific and highly sensitive diagnostic tools. In this work, the root RNA-virome of a Solanum phureja cv. Criolla Colombia plant was characterized using 454 GS-FLX pyrosequencing. Co-infection with three different viruses was found, the most prevalent of which were identified as two variants of Potato virus S: PVSCol (49.7 %) and PVSA (42.7 %). The remaining viruses corresponded to Potato virus V (PVV) (6.5 %) and Potato leafroll virus (PLRV) (1.1 %). At least two genomic variants were found for each virus, an important factor that should be taken into account in the design of molecular detection tools supporting seed certification and genetic improvement programs.