Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/10495/28302
Título : Enhanced control of Mycobacterium Tuberculosis Extrapulmonary Dissemination in Mice by an Arabinomannan-protein Conjugate Vaccine
Autor : Baena García, Andrés
Prados Rosales, Rafael
Carreño, Leandro
Cheng, Tingting
Blanc, Caroline
Weinrick, Brian
Malek, Adel
Lowary, Todd L.
metadata.dc.subject.*: Tuberculosis
Mycobacterium tuberculosis
Vaccines, Conjugate
Vacunas Conjugadas
Fecha de publicación : 2017
Editorial : Public Library of Science
Resumen : ABSTRACT: Currently there are a dozen or so of new vaccine candidates in clinical trials for prevention of tuberculosis (TB) and each formulation attempts to elicit protection by enhancement of cellmediated immunity (CMI). In contrast, most approved vaccines against other bacterial pathogens are believed to mediate protection by eliciting antibody responses. However, it has been difficult to apply this formula to TB because of the difficulty in reliably eliciting protective antibodies. Here, we developed capsular polysaccharide conjugates by linking mycobacterial capsular arabinomannan (AM) to either Mtb Ag85b or B. anthracis protective antigen (PA). Further, we studied their immunogenicity by ELISA and AM glycan microarrays and protection efficacy in mice. Immunization with either Abg85b-AM or PA-AM conjugates elicited an AM-specific antibody response in mice. AM binding antibodies stimulated transcriptional changes in Mtb. Sera from AM conjugate immunized mice reacted against a broad spectrum of AM structural variants and specifically recognized arabinan fragments. Conjugate vaccine immunized mice infected with Mtb had lower bacterial numbers in lungs and spleen, and lived longer than control mice. These findings provide additional evidence that humoral immunity can contribute to protection against Mtb.
metadata.dc.identifier.eissn: 1553-7374
ISSN : 1553-7366
metadata.dc.identifier.doi: 10.1371/journal.ppat.1006250
Aparece en las colecciones: Artículos de Revista en Ciencias Médicas

Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
BaenaAndres_2017_f MycobacteriumTuberculosis.pdfArtículo de investigación5.16 MBAdobe PDFVisualizar/Abrir


Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons Creative Commons