Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/10495/32135
Título : Endothelial activation and injury by microparticles in patients with systemic lupus erythematosus and rheumatoid arthritis
Autor : Atehortúa Castro, Laura Melissa
Rojas López, Mauricio
Vásquez Duque, Gloria María
Muñoz Vahos, Carlos Horacio
Posada Duque, Rafael Andrés
Castaño Monsalve, Diana María
metadata.dc.subject.*: Lupus Eritematoso Sistémico
Lupus Erythematosus, Systemic
Antígenos CD
Antigens, CD
Artritis Reumatoide
Arthritis, Rheumatoid
Células Endoteliales
Endothelial Cells
Cadherinas
Cadherins
Micropartículas Derivadas de Células
Cell-Derived Microparticles
Fecha de publicación : 2019
Editorial : BMC (Biomed Central)
Citación : Atehortúa L, Rojas M, Vásquez G, Muñoz-Vahos CH, Vanegas-García A, Posada-Duque RA, Castaño D. Endothelial activation and injury by microparticles in patients with systemic lupus erythematosus and rheumatoid arthritis. Arthritis Res Ther. 2019 Jan 23;21(1):34. doi: 10.1186/s13075-018-1796-4.
Resumen : ABSTRACT: Background: Endothelial activation and damage is commonly observed in patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) and is related to development of atherosclerosis and cardiovascular diseases. Different components of the immune system seem to participate in the endothelial injury, such as generation of autoantibodies and formation of immune complexes (ICs). Microparticles (MPs) and their immune complexes (MPs-ICs) are increased in the circulation of patients with SLE and RA; therefore, we propose these extracellular vesicles could interact and modulate the function of endothelial cells. Hence, the effect of MPs and MPs-ICs from patients with SLE and RA in endothelial cells was evaluated. Methods: Macrovascular and microvascular endothelial cells were exposed to MPs and MPs-ICs from healthy donors and patients with SLE and RA. Vesicles uptake/binding, expression of adhesion molecules, cytokine and chemokine production, monocyte adherence, and alterations of endothelial monolayer were evaluated by flow cytometry and fluorescence microscopy. Results: Endothelial cells internalized MPs and MPs-ICs and increased CD54 and CD102 expression and CCL2, CCL5, and IL-6 production after the treatment with these extracellular vesicles, which led to an increase in the adherence of classic monocytes. These vesicles also induced low expression of VE-cadherin in membrane, depolymerization of actin filaments, and formation of intercellular spaces, which led to endothelial death and increased permeability after MPs and MPs-ICs exposure. Conclusions: MPs and MPs-ICs from patients with SLE and RA increase adhesion molecules expression, chemokine production, and structural alterations in macrovascular and microvascular endothelial cells. Therefore, high counts of these vesicles in patients would promote endothelial alterations and secondary tissue leukocyte infiltration.
metadata.dc.identifier.eissn: 1478-6362
ISSN : 1478-6354
metadata.dc.identifier.doi: 10.1186/s13075-018-1796-4
Aparece en las colecciones: Artículos de Revista en Ciencias Médicas

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