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dc.contributor.authorRuiz Cortés, Zulma Tatiana-
dc.contributor.authorMartel Kennes, Yan-
dc.contributor.authorGévry, Nicolas Y.-
dc.contributor.authorDowney, Bruce R.-
dc.contributor.authorPalin, Marie France-
dc.contributor.authorMurphy, Bruce D.-
dc.date.accessioned2023-02-07T02:24:51Z-
dc.date.available2023-02-07T02:24:51Z-
dc.date.issued2003-
dc.identifier.citationRuiz-Cortés ZT, Martel-Kennes Y, Gévry NY, Downey BR, Palin MF, Murphy BD. Biphasic effects of leptin in porcine granulosa cells. Biol Reprod. 2003 Mar;68(3):789-96. doi: 10.1095/biolreprod.102.010702.spa
dc.identifier.issn0006-3363-
dc.identifier.urihttps://hdl.handle.net/10495/33389-
dc.description.abstractABSTRACT: The direct effects of recombinant porcine leptin on porcine granulosa cells were studied to test the hypothesis that leptin, acting through the nuclear transcription factor signal transducer and activator of transcription 3 (STAT-3), modulates sterol regulatory element-binding protein 1 (SREBP1) thereby increasing steroidogenesis. In porcine granulosa cells in culture over 48 h, leptin at 10 ng/ml increased progesterone accumulation 3-fold while it was reduced by leptin at 1000 ng/ml. Leptin had no effect on progression of granulosa cells through the cell cycle nor on the frequency of cell death. Leptin treatment at 24 or 48 h of culture resulted in dose-dependent 2- to 4-fold increases in tyrosine phosphorylation of STAT-3. Leptin had a biphasic effect on the abundance of membrane-bound and transcriptionally active forms of SREBP1. In transient transfection of primary porcine granulosa cells, the plasmid expressing the transcriptionally active form of SREPB-1 induced transcription of the key regulator of steroidogenesis, the steroidogenic acute regulatory protein (StAR). StAR transcription was also increased by the low dose of leptin and was further upregulated in the presence of the SREBP plasmid. Leptin at 1000 ng/ml inhibited SREBP1-induced StAR expression. Thus, leptin, acting through STAT-3, modulates steroidogenesis in a biphasic and dose-dependent manner, and SREBP1 induction of StAR expression may be in the cascade of regulatory events.spa
dc.format.extent7spa
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherSociety for the Study of Reproductionspa
dc.publisherOxford University Pressspa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersionspa
dc.rightsinfo:eu-repo/semantics/openAccessspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/2.5/co/*
dc.titleBiphasic Effects of Leptin in Porcine Granulosa Cellsspa
dc.typeinfo:eu-repo/semantics/articlespa
dc.publisher.groupBiogénesisspa
dc.identifier.doi10.1095/biolreprod.102.010702.-
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2spa
dc.identifier.eissn1529-7268-
oaire.citationtitleBiology of Reproducionspa
oaire.citationstartpage789spa
oaire.citationendpage796spa
oaire.citationvolume68spa
oaire.citationissue3spa
dc.rights.creativecommonshttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.publisher.placeNueva York, Estados Unidosspa
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1spa
dc.type.redcolhttps://purl.org/redcol/resource_type/ARTspa
dc.type.localArtículo de investigaciónspa
dc.subject.decsCuerpo Lúteo-
dc.subject.decsCorpus Luteum-
dc.subject.decsLeptina-
dc.subject.decsLeptin-
dc.subject.decsReceptores de Leptina-
dc.subject.decsReceptors, Leptin-
dc.subject.decsProgesterona-
dc.subject.decsProgesterone-
dc.subject.decsProteínas Potenciadoras de Unión a CCAAT-
dc.subject.decsCCAAT-Enhancer-Binding Proteins-
dc.subject.decsCélulas de la Granulosa-
dc.subject.decsGranulosa Cells-
dc.subject.decsPorcinos-
dc.subject.decsSwine-
dc.subject.decsProteínas de Unión al ADN-
dc.subject.decsDNA-Binding Proteins-
dc.subject.decsCélulas Cultivadas-
dc.subject.decsCells, Cultured-
dc.subject.decsTranscripción Genética-
dc.subject.decsTranscription, Genetic-
dc.description.researchgroupidCOL0066561spa
dc.relation.ispartofjournalabbrevBiol. Reprod.spa
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