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Título : | Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon |
Autor : | Vélez Bernal, Iván Darío Ruiz López, John Freddy Linton, Yvonne Marie Quiñones Pinzón, Martha Lucía Wilkerson, Richard C Ponsonby, David J Conn, Jan Herrera Varela, Manuela |
metadata.dc.subject.*: | Anopheles ADN Intergénico - genética DNA, Intergenic - genetics ADN Mitocondrial - genética DNA, Mitochondrial - genetics ADN Ribosómico - genética DNA, Ribosomal - genetics ADN Espaciador Ribosómico - genética DNA, Ribosomal Spacer - genetics Complejo IV de Transporte de Electrones - genética Electron Transport Complex IV - genetics Análisis de Secuencia de ADN Sequence Analysis, DNA Especificidad de la Especie Species Specificity Brasil Brazil Colombia Colombia https://id.nlm.nih.gov/mesh/D000852 https://id.nlm.nih.gov/mesh/D021901 https://id.nlm.nih.gov/mesh/D004272 https://id.nlm.nih.gov/mesh/D004275 https://id.nlm.nih.gov/mesh/D021903 https://id.nlm.nih.gov/mesh/D003576 https://id.nlm.nih.gov/mesh/D017422 https://id.nlm.nih.gov/mesh/D013045 https://id.nlm.nih.gov/mesh/D001938 https://id.nlm.nih.gov/mesh/D003105 |
Fecha de publicación : | 2010 |
Editorial : | Instituto Oswaldo Cruz |
Citación : | Ruiz F, Linton YM, Ponsonby DJ, Conn JE, Herrera M, Quiñones ML, Vélez ID, Wilkerson RC. Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon. Mem Inst Oswaldo Cruz. 2010 Nov;105(7):899-903. doi: 10.1590/s0074-02762010000700010. PMID: 21120360; PMCID: PMC4438775. |
Resumen : | ABSTRACT: The presence of Anopheles (Nyssorhynchus) dunhami Causey in Colombia (Department of Amazonas) is confirmed for the first time through direct comparison of mtDNA cytochrome c oxidase I (COI) barcodes and nuclear rDNA second internal transcribed spacer (ITS2) sequences with topotypic specimens of An. dunhami from Tefé, Brazil. An. dunhami was identified through retrospective correlation of DNA sequences following misidentification as Anopheles nuneztovari s.l. using available morphological keys for Colombian mosquitoes. That An. dunhami occurs in Colombia and also possibly throughout the Amazon Basin, is of importance to vector control programs, as this non-vector species is morphologically similar to known malaria vectors including An. nuneztovari, Anopheles oswaldoi and Anopheles trinkae. Species identification of An. dunhami and differentiation from these closely related species are highly robust using either DNA ITS2 sequences or COI DNA barcode. DNA methods are advocated for future differentiation of these often sympatric taxa in South America. |
metadata.dc.identifier.eissn: | 1678-8060 |
ISSN : | 0074-0276 |
metadata.dc.identifier.doi: | 10.1590/s0074-02762010000700010 |
Aparece en las colecciones: | Artículos de Revista en Ciencias Médicas |
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VelezIvan_2010_Molecular_Topotypic_Colombia.pdf | Artículo de investigación | 562.47 kB | Adobe PDF | Visualizar/Abrir |
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