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Título : Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon
Autor : Vélez Bernal, Iván Darío
Ruiz López, John Freddy
Linton, Yvonne Marie
Quiñones Pinzón, Martha Lucía
Wilkerson, Richard C
Ponsonby, David J
Conn, Jan
Herrera Varela, Manuela
metadata.dc.subject.*: Anopheles
ADN Intergénico - genética
DNA, Intergenic - genetics
ADN Mitocondrial - genética
DNA, Mitochondrial - genetics
ADN Ribosómico - genética
DNA, Ribosomal - genetics
ADN Espaciador Ribosómico - genética
DNA, Ribosomal Spacer - genetics
Complejo IV de Transporte de Electrones - genética
Electron Transport Complex IV - genetics
Análisis de Secuencia de ADN
Sequence Analysis, DNA
Especificidad de la Especie
Species Specificity
Brasil
Brazil
Colombia
Colombia
https://id.nlm.nih.gov/mesh/D000852
https://id.nlm.nih.gov/mesh/D021901
https://id.nlm.nih.gov/mesh/D004272
https://id.nlm.nih.gov/mesh/D004275
https://id.nlm.nih.gov/mesh/D021903
https://id.nlm.nih.gov/mesh/D003576
https://id.nlm.nih.gov/mesh/D017422
https://id.nlm.nih.gov/mesh/D013045
https://id.nlm.nih.gov/mesh/D001938
https://id.nlm.nih.gov/mesh/D003105
Fecha de publicación : 2010
Editorial : Instituto Oswaldo Cruz
Citación : Ruiz F, Linton YM, Ponsonby DJ, Conn JE, Herrera M, Quiñones ML, Vélez ID, Wilkerson RC. Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon. Mem Inst Oswaldo Cruz. 2010 Nov;105(7):899-903. doi: 10.1590/s0074-02762010000700010. PMID: 21120360; PMCID: PMC4438775.
Resumen : ABSTRACT: The presence of Anopheles (Nyssorhynchus) dunhami Causey in Colombia (Department of Amazonas) is confirmed for the first time through direct comparison of mtDNA cytochrome c oxidase I (COI) barcodes and nuclear rDNA second internal transcribed spacer (ITS2) sequences with topotypic specimens of An. dunhami from Tefé, Brazil. An. dunhami was identified through retrospective correlation of DNA sequences following misidentification as Anopheles nuneztovari s.l. using available morphological keys for Colombian mosquitoes. That An. dunhami occurs in Colombia and also possibly throughout the Amazon Basin, is of importance to vector control programs, as this non-vector species is morphologically similar to known malaria vectors including An. nuneztovari, Anopheles oswaldoi and Anopheles trinkae. Species identification of An. dunhami and differentiation from these closely related species are highly robust using either DNA ITS2 sequences or COI DNA barcode. DNA methods are advocated for future differentiation of these often sympatric taxa in South America.
metadata.dc.identifier.eissn: 1678-8060
ISSN : 0074-0276
metadata.dc.identifier.doi: 10.1590/s0074-02762010000700010
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