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Título : Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection
Autor : Barrera Robledo, Luis Fernando
Alzate Restrepo, Juan Fernando
López Agudelo, Víctor Alonso
Cabarcas Jaramillo, Felipe
Barrera Enríquez, Vianey Paola
Ríos Estepa, Rigoberto
Baena García, Andrés
metadata.dc.subject.*: Interacciones Huésped-Patógeno
Host-Pathogen Interactions
Mycobacterium tuberculosis
Macrófagos
Macrophages
RNA-Seq
RNA-Seq
Tuberculosis
https://id.nlm.nih.gov/mesh/D054884
https://id.nlm.nih.gov/mesh/D009169
https://id.nlm.nih.gov/mesh/D008264
https://id.nlm.nih.gov/mesh/D000081246
https://id.nlm.nih.gov/mesh/D014376
Fecha de publicación : 2022
Editorial : MDPI
Citación : López-Agudelo VA, Baena A, Barrera V, Cabarcas F, Alzate JF, Beste DJV, Ríos-Estepa R, Barrera LF. Dual RNA Sequencing of Mycobacterium tuberculosis-Infected Human Splenic Macrophages Reveals a Strain-Dependent Host-Pathogen Response to Infection. Int J Mol Sci. 2022 Feb 4;23(3):1803. doi: 10.3390/ijms23031803.
Resumen : ABSTRACT: Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb), leading to pulmonary and extrapulmonary TB, whereby Mtb is disseminated to many other organs and tissues. Dissemination occurs early during the disease, and bacteria can be found first in the lymph nodes adjacent to the lungs and then later in the extrapulmonary organs, including the spleen. The early global gene expression response of human tissue macrophages and intracellular clinical isolates of Mtb has been poorly studied. Using dual RNA-seq, we have explored the mRNA profiles of two closely related clinical strains of the Latin American and Mediterranean (LAM) family of Mtb in infected human splenic macrophages (hSMs). This work shows that these pathogens mediate a distinct host response despite their genetic similarity. Using a genome-scale host-pathogen metabolic reconstruction to analyze the data further, we highlight that the infecting Mtb strain also determines the metabolic response of both the host and pathogen. Thus, macrophage ontogeny and the genetic-derived program of Mtb direct the host-pathogen interaction.
metadata.dc.identifier.eissn: 1422-0068
ISSN : 1661-6596
metadata.dc.identifier.doi: 10.3390/ijms23031803
Aparece en las colecciones: Artículos de Revista en Ciencias Médicas

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