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https://hdl.handle.net/10495/40661| Título : | Venomic, transcriptomic, and bioactivity analyses of pamphobeteus verdolaga venom reveal complex disulfide-rich peptides that modulate calcium channels |
| Autor : | Estrada Gómez, Sebastián Vargas Muñoz, Leidy Johana Quintana Castillo, Juan Carlos Caldas Cardoso, Fernanda Arenas Gómez, Claudia Marcela Pineda, Sandy Steffany Saldarriaga Córdoba, Mónica María |
| metadata.dc.subject.*: | Secuencia de Aminoácidos Amino Acid Sequence Bloqueadores de los Canales de Calcio - aislamiento & purificación Calcium Channel Blockers - isolation & purification Bloqueadores de los Canales de Calcio - farmacología Calcium Channel Blockers - pharmacology Canales de Calcio - metabolismo Calcium Channels - metabolism Línea Celular Tumoral Cell Line, Tumor Disulfuros - aislamiento & purificación Disulfides - isolation & purification Disulfuros - farmacología Disulfides - pharmacology Anotación de Secuencia Molecular Molecular Sequence Annotation Péptidos Peptides Alineación de Secuencia Sequence Alignment Venenos de Araña - química Spider Venoms - chemistry Venenos de Araña - genética Spider Venoms - genetics Arañas - genética Spiders - genetics Transcriptoma - genética Transcriptome - genetics https://id.nlm.nih.gov/mesh/D000595 https://id.nlm.nih.gov/mesh/D002121 https://id.nlm.nih.gov/mesh/D015220 https://id.nlm.nih.gov/mesh/D045744 https://id.nlm.nih.gov/mesh/D004220 https://id.nlm.nih.gov/mesh/D058977 https://id.nlm.nih.gov/mesh/D010455 https://id.nlm.nih.gov/mesh/D016415 https://id.nlm.nih.gov/mesh/D013111 https://id.nlm.nih.gov/mesh/D013112 https://id.nlm.nih.gov/mesh/D059467 |
| Fecha de publicación : | 2019 |
| Editorial : | MDPI |
| Citación : | Estrada-Gomez S, Cardoso FC, Vargas-Muñoz LJ, Quintana-Castillo JC, Arenas Gómez CM, Pineda SS, Saldarriaga-Cordoba MM. Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels. Toxins (Basel). 2019 Aug 27;11(9):496. doi: 10.3390/toxins11090496. |
| Resumen : | ABSTRACT: Pamphobeteus verdolaga is a recently described Theraphosidae spider from the Andean region of Colombia. Previous reports partially characterized its venom profile. In this study, we conducted a detailed analysis that includes reversed-phase high-performance liquid chromatography (rp-HPLC), calcium influx assays, tandem mass spectrometry analysis (tMS/MS), and venom-gland transcriptome. rp-HPLC fractions of P. verdolaga venom showed activity on CaV2.2, CaV3.2, and NaV1.7 ion channels. Active fractions contained several peptides with molecular masses ranging from 3399.4 to 3839.6 Da. The tMS/MS analysis of active fraction displaying the strongest activity to inhibit calcium channels showed sequence fragments similar to one of the translated transcripts detected in the venom-gland transcriptome. The putative peptide of this translated transcript corresponded to a toxin, here named ω-theraphositoxin-Pv3a, a potential ion channel modulator toxin that is, in addition, very similar to other theraphositoxins affecting calcium channels (i.e., ω-theraphotoxin-Asp1a). Additionally, using this holistic approach, we found that P. verdolaga venom is an important source of disulfide-rich proteins expressing at least eight superfamilies. |
| metadata.dc.identifier.eissn: | 2072-6651 |
| metadata.dc.identifier.doi: | 10.3390/toxins11090496 |
| Aparece en las colecciones: | Artículos de Revista en Farmacéutica y Alimentarias |
Ficheros en este ítem:
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| GómezSebastian_2019_Venomic_Transcriptomic_Bioactivity.epub | Artículo de investigación | 20.04 MB | EPUB | Visualizar/Abrir |
| GómezSebastian_2019_Venomic_Transcriptomic_Bioactivity.pdf | Artículo de investigación | 6.5 MB | Adobe PDF | Visualizar/Abrir |
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