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Título : Genotoxicidad sobre linfocitos humanos expuestos a PM10 de tres sitios del Valle de Aburrá (Antioquia)
Otros títulos : Genotoxicity in human lymphocytes exposed to PM10 from three sites in the Valle de Aburrá (Antioquia)
Autor : Mendoza Zapata, Lady Carolina
Orozco Jiménez, Luz Yaneth
Zapata Restrepo, Lina María
Palacio Baena, Jaime Alberto
metadata.dc.subject.*: Biomarcadores
Contaminación ambiental
Electroforesis alcalina de células individuales
Ensayo cometa
Genotoxicidad
Linfocitos
Material particulado
Fecha de publicación : 2013
Editorial : Universidad Nacional de Colombia, Facultad de Medicina
Citación : Mendoza Zapata, L. C., Orozco Jiménez, L. Y., Zapata Restrepo, L. M., & Palacio Baena, J. A. (2013). Genotoxicidad sobre linfocitos humanos expuestos a PM10 de tres sitios del Valle de Aburrá (Antioquia). Revista de Salud Publica. 15(2), 294-306.
Resumen : Objective Assessing air quality by determining PM10 genotoxicity in human lymphocytes at three locations in the Valle de Aburrá (Antioquia department). Methods Three sites were chosen in the Valle de Aburrá (Barbosa, Corantioquia and the School of Mines) using Colombian reference (50 g/m3) and PM10 content values, having annual low (25 mg /m3), medium (44 ug/m3) and high PM10 average (91 ug/m3). PM10 filters were analyzed during three different seasons between 2011and 2012: rainy, transitional and dry. Human lymphocytes were treated with the organic extract obtained from each filter to evaluate DNA damage using an alkaline comet assay. Results Genotoxicity was found to be highly significant (p<0.001) in all cases, compared to the negative control. The highest damage (six times) was seen in material from the School of Mines and during the transitional period. Differences were found between material from Barbosa and Corantioquia regarding that from the School of Mines concerning the induction of damage. Conclusions Although the PM10 values reported for Barbosa and Corantioquia were below the maximum permitted level, genotoxic activity was found for PM10 from both sites as well as for the School of Mines. These results show that physical- chemical monitoring of particulate matter is not enough for assessing the exposed population’s relative risk. Such analysis should thus be accompanied by using genotoxicity biomarkers, such as the comet assay.
ISSN : 01240064
25393596
metadata.dc.identifier.doi: 10.15446/rsap
Aparece en las colecciones: CIA (Centro de Investigaciones Ambientales y de Ingeniería)

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