Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/10495/22045
Título : Identification and Analysis of the Role of Superoxide Dismutases Isoforms in the Pathogenesis of Paracoccidioides spp.
Autor : Tamayo Ossa, Diana Patricia
Muñoz Gómez, José Fernando
López García, Ángela María
Urán Jiménez, Martha Eugenia
Herrera Noreña, Juan Sebastián
Borges, Clayton Luiz
Restrepo Moreno, Ángela
De Almeida Soares, Célia Maria
Taborda, Carlos
Almeida Ramalho, Agostinho Joao
McEwen Ochoa, Juan Guillermo
Hernández Ruiz, Orville
metadata.dc.subject.*: Superóxido Dismutasa
Superoxide Dismutase
Paracoccidioides
Isoformas de Proteínas
Protein Isoforms
Patogénesis
Pathogenesis
http://aims.fao.org/aos/agrovoc/c_24881
Fecha de publicación : 2016
Editorial : Public Library of Science
Resumen : ABSTRACT: The ability of Paracoccidioides to defend itself against reactive oxygen species (ROS) produced by host effector cells is a prerequisite to survive. To counteract these radicals, Paracoccidioides expresses, among different antioxidant enzymes, superoxide dismutases (SODs). In this study, we identified six SODs isoforms encoded by the Paracoccidioidesgenome. We determined gene expression levels of representative isolates of the phylogenetic lineages of Paracoccidioides spp. (S1, PS2, PS3 and Pb01-like) using quantitative RT-PCR. Assays were carried out to analyze SOD gene expression of yeast cells, mycelia cells, the mycelia-to-yeast transition and the yeast-to-mycelia germination, as well as under treatment with oxidative agents and during interaction with phagocytic cells. We observed an increased expression of PbSOD1 and PbSOD3 during the transition process, exposure to oxidative agents and interaction with phagocytic cells, suggesting that these proteins could assist in combating the superoxide radicals generated during the host-pathogen interaction. Using PbSOD1 and PbSOD3 knockdown strains we showed these genes are involved in the response of the fungus against host effector cells, particularly the oxidative stress response, and in a mouse model of infection. Protein sequence analysis together with functional analysis of knockdown strains seem to suggest that PbSOD3 expression islinked with a pronounced extracellular activity while PbSOD1 seems more related to intra cellular requirements of the fungus. Altogether, our data suggests that P. brasiliensis actively responds to the radicals generated endogenously during metabolism and counteracts the oxidative burst of immune cells by inducing the expression of SOD isoforms.
metadata.dc.identifier.eissn: 1935-2735
ISSN : 1935-2727
metadata.dc.identifier.doi: 10.1371/journal.pntd.0004481
Aparece en las colecciones: Artículos de Revista en Microbiología

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