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https://hdl.handle.net/10495/23665
Título : | A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae) |
Autor : | Camargo Guerrero, Mauricio Duque Correa, María Adelaida Berrío, Alejandro |
metadata.dc.subject.*: | Triatominae Cromosoma Chromosomes Microdifusión http://aims.fao.org/aos/agrovoc/c_1598 https://id.nlm.nih.gov/mesh/D014225 |
Fecha de publicación : | 2006 |
Editorial : | Instituto Oswaldo Cruz |
Resumen : | ABSTRACT: Cytogenetics of triatomines have been a valuable biological tool for the study of evolution, taxonomy, and epidemiology of these vectors of Trypanosoma cruzi. Here we present a single microtube protocol that combines micro-centrifugation and micro-spreading, allowing high quality cytogenetic preparations from male gonadal material of Rhodnius prolixus and Triatoma lecticularia. The amount of cellular scattering can be modulated, which can be useful if small aggregates of synchronous cells are desired. Moreover, a higher number of slides per gonad can be obtained with fully flattened clean chromosomal spreads with minimum overlaps, optimal for classical and modern molecular cytogenetic analyses. |
metadata.dc.identifier.eissn: | 1678-8060 |
ISSN : | 0074-0276 |
metadata.dc.identifier.doi: | 10.1590/S0074-02762006000300021 |
Aparece en las colecciones: | Artículos de Revista en Ciencias Exactas y Naturales |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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CamargoMauricio_2006_MicroSpreadingSpermatogenic.pdf | Artículo de investigación | 427.19 kB | Adobe PDF | Visualizar/Abrir |
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