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dc.contributor.authorPenagos Tabares, Felipe-
dc.contributor.authorLange, Malin K-
dc.contributor.authorSeipp, Anika-
dc.contributor.authorGärtner, Ulrich-
dc.contributor.authorMejer, Helena-
dc.contributor.authorTaubert, Anja-
dc.contributor.authorHermosilla, Carlos-
dc.date.accessioned2023-10-29T20:26:25Z-
dc.date.available2023-10-29T20:26:25Z-
dc.date.issued2018-
dc.identifier.citationPenagos-Tabares, F., Lange, M. K., Seipp, A., Gärtner, U., Mejer, H., Taubert, A., & Hermosilla, C. (2018). Novel approach to study gastropod-mediated innate immune reactions against metastrongyloid parasites. Parasitology research, 117(4), 1211–1224. https://doi.org/10.1007/s00436-018-5803-0spa
dc.identifier.issn0932-0113-
dc.identifier.urihttps://hdl.handle.net/10495/37100-
dc.description.abstractABSTRACT: The anthropozoonotic metastrongyloid nematodes Angiostrongylus cantonensis and Angiostrongylus costaricensis, as well as Angiostrongylus vasorum, Crenosoma vulpis, Aelurostrongylus abstrusus and Troglostrongylus brevior are currently considered as emerging gastropod-borne parasites and have gained growing scientific attention in the last years. However, the knowledge on invertebrate immune responses and on how metastrongyloid larvae are attacked by gastropod immune cells is still limited. This work aims to describe an in vitro system to investigate haemocyte-derived innate immune responses of terrestrial gastropods induced by vital axenic metastrongyloid larvae. We also provide protocols on slug/snail management and breeding under standardized climate conditions (circadian cycle, temperature and humidity) for the generation of parasite-free F0 stages which are essential for immune-related investigations. Adult slug species (Arion lusitanicus, Limax maximus) and giant snails (Achatina fulica) were maintained in fully automated climate chambers until mating and production of fertilized eggs. Newly hatched F0 juvenile specimens were kept under parasite-free conditions before experimental use. An improved protocol for gastropod haemolymph collection and haemocyte isolation was established. Giemsa-stained haemolymph preparations showed adequate haemocyte isolation in all three gastropod species. Additionally, a protocol for the production of axenic first and third stage larvae (L1, L3) was established. Haemocyte functionality was tested in haemocyte-nematode-co-cultures. Scanning electron microscopy (SEM) and light microscopy analyses revealed that gastropodderived haemocytes formed clusters as well as DNA-rich extracellular aggregates catching larvae and decreasing their motility. These data confirm the usefulness of the presented methods to study haemocyte-mediated gastropod immune responses to better understand the complex biology of gastropod-borne diseases.spa
dc.format.extent14spa
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherSpringerspa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersionspa
dc.rightsinfo:eu-repo/semantics/openAccessspa
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/co/*
dc.titleNovel approach to study gastropod-mediated innate immune reactions against metastrongyloid parasitesspa
dc.typeinfo:eu-repo/semantics/articlespa
dc.publisher.groupCentro de Investigaciones Básicas y Aplicadas en Veterinaria (CIBAV)spa
dc.identifier.doi10.1007/s00436-018-5803-0-
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.rights.accessrightshttp://purl.org/coar/access_right/c_abf2spa
dc.identifier.eissn1432-1955-
oaire.citationtitleParasitology Researchspa
oaire.citationstartpage1211spa
oaire.citationendpage1224spa
oaire.citationvolume117spa
oaire.citationissue4spa
dc.rights.creativecommonshttps://creativecommons.org/licenses/by/4.0/spa
dc.publisher.placeBerlín, Alemaniaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1spa
dc.type.redcolhttps://purl.org/redcol/resource_type/ARTspa
dc.type.localArtículo de investigaciónspa
dc.subject.decsHemocitos - inmunología-
dc.subject.decsHemocytes - immunology-
dc.subject.decsMetastrongyloidea-
dc.subject.decsAngiostrongylus / immunology*-
dc.subject.decsInmunidad Innata-
dc.subject.decsImmunity, Innate-
dc.subject.decsMicroscopía Electrónica de Rastreo-
dc.subject.decsMicroscopy, Electron, Scanning-
dc.subject.decsParásitos-
dc.subject.decsParasites-
dc.subject.decsCaracoles-
dc.subject.decsSnails-
dc.subject.decsInfecciones por Strongylida-
dc.subject.decsStrongylida Infections-
dc.description.researchgroupidCOL0153246spa
dc.relation.ispartofjournalabbrevParasitol. Res.spa
Aparece en las colecciones: Artículos de Revista en Ciencias Agrarias

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